Mutation ofBAX occurs infrequently in acquired immunodeficiency syndrome-related non-Hodgkin's lymphomas

Autor: Gian Carlo Avanzi, Annunziata Gloghini, Luigi Maria Larocca, Margherita Gallicchio, Daniela Capello, Daniela Vivenza, Antonella Cingolani, Marina I. Gutiérrez, Daniela Buonaiuto, Antonino Carbone, Gianluca Gaidano, Giuseppe Saglio, Riccardo Dalla-Favera, Cristiano Ariatti, Kishor Bhatia, Francesco Forconi, Umberto Tirelli, Lucia Fassone
Rok vydání: 2000
Předmět:
Zdroj: Genes, Chromosomes and Cancer. 27:177-182
ISSN: 1098-2264
1045-2257
DOI: 10.1002/(sici)1098-2264(200002)27:2<177::aid-gcc9>3.0.co;2-o
Popis: Acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (AIDS-NHLs) consistently derive from B cells, are histologically heterogeneous, and are associated with distinct molecular pathways depending upon histology. Recently, it has been proposed that inactivating mutations of the bax death agonist may contribute to the pathogenesis of human tumors. In particular, among B-cell malignancies, BAX mutations have been detected at a certain frequency in Burkitt lymphomas. This study is aimed at defining the status of the BAX gene throughout the clinicopathologic spectrum of AIDS-NHL (n = 54), including AIDS-related Burkitt lymphoma (n = 14), AIDS-related Burkitt-like lymphoma (n = 8), AIDS-related diffuse large cell lymphoma (n = 15), AIDS-related primary central nervous system lymphoma (n = 6), and AIDS-related primary effusion lymphoma (n = 11). All 6 BAX exons and flanking sequences were subjected to mutational analysis by polymerase chain reaction-single strand conformation polymorphism followed by DNA direct sequencing of positive cases. Mutations of BAX among AIDS-NHL were restricted to a cell line of AIDS-related primary effusion lymphoma, which harbored a frameshift mutation causing the introduction of a proximal stop codon. All other AIDS-NHL displayed wild-type BAX alleles. In order to investigate whether BAX inactivation in AIDS-NHL may occur through mechanisms other than gene mutation, bax protein expression was investigated by Western blot analysis or immunohistochemistry in selected cases. All AIDS-NHL analyzed expressed normal bax proteins. Overall, this study indicates that deregulation of apoptotic control in AIDS-NHL is not caused by BAX alterations. Genes Chromosomes Cancer 27:177-182, 2000.
Databáze: OpenAIRE
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