Delineating the organization of projection neuron subsets in primary visual cortex with multiple fluorescent rabies virus tracing

Autor: Xiangwei Shi, Liang Li, Hannah C. Webber, Zhenfang Fu, Xiangning Li, Khaista Rahman, Jinxia Dai, Hui Gong, Jinsong Yu, Gang Cao, Zhe Hu, Xiaoyu Zhang, Siheng Zhang, Leqiang Sun, Yajie Tang
Rok vydání: 2021
Předmět:
Zdroj: Brain Structure and Function. 226:951-961
ISSN: 1863-2661
1863-2653
DOI: 10.1007/s00429-021-02250-7
Popis: The impressive functions of the brain rely on an extensive connectivity matrix between specific neurons, the architecture of which is frequently characterized by one brain nucleus/region connecting to multiple targets, either via collaterals of the same projection neuron or several, differentially specified neurons. Delineating the fine architecture of projection neuron subsets in a specific brain region could greatly facilitate its circuit, computational, and functional resolution. Here, we developed multiple fluorescent rabies viruses (RV) to delineate the fine organization of corticothalamic projection neuron subsets in the primary visual cortex (V1). By simultaneously retrograde labeling multiple distinct subsets of corticothalamic projection neurons in V1 from their target nuclei in thalamus (dLGN, LP, LD), we observed that V1-dLGN corticothalamic projection neurons were densely concentrated in layer VI, except for several sparsely scattered neurons in layer V, while V1-LP and V1-LD corticothalamic projection neurons were localized to both layers V and VI. Meanwhile, we observed a fraction of V1 corticothalamic projection neurons targeting two thalamic nuclei, which was further confirmed by fMOST whole-brain imaging. The multiple fluorescent RV tracing tools can be extensively applied to resolve the architecture of projection neuron subsets in certain brain regions, with a strong potential to delineate the computational and functional organization of these brain regions.
Databáze: OpenAIRE
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