Kinetic ethylene glycol assay with use of yeast alcohol dehydrogenase
| Autor: | J H Eckfeldt, R T Light |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Ethanol
Chromatography biology medicine.diagnostic_test Biochemistry (medical) Clinical Biochemistry Kinetics Alcohol oxidoreductase medicine.disease chemistry.chemical_compound chemistry Ethylene glycol poisoning Spectrophotometry biology.protein medicine Methanol Ethylene glycol Alcohol dehydrogenase |
| Zdroj: | Clinical Chemistry. 26:1278-1280 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | We describe a rapid, accurate, and precise two-point kinetic assay for ethylene glycol. The method involves use of a standard kit for ethanol determination with yeast alcohol dehydrogenase, and of a centrifugal analyzer. Alcohol dehydrogenase catalyzes the oxidation of ethylene glycol in a trichloroacetic acid-precipitated specimen; the resulting NADH production is monitored at 340 nm. The reaction rate is linear with concentration to 1.5 g of ethylene glycol per liter. Interference from methanol, ethanol, and isopropanol was easily recognized after a 30-min incubation at 100 degrees C. We believe that the method can be readily adaptable to any narrow-bandwidth, stable, temperature-controlled spectrophotometer and so should provide more widely for the prompt assessment of patients in whom ethylene glycol poisoning is suspected. |
| Databáze: | OpenAIRE |
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