Autor:	Jason D Limberis
Rok vydání:	2022
DOI:	10.17504/protocols.io.36wgqj9zyvk5/v1
Popis:	The ligation of dumbbell (hairpin) oligos to linear dsDNA produces pseudo-circular DNA. Including deoxyUridine in the PCR primer sequences causes Q5 and other high-fidelity polymerases to arrest elongation. This results in overhangs that were successfully ligated to a complementary hairpin structure. The deoxyUridine reduced the PCR product by approximately two-thirds, but this was ameliorated by increasing the Q5 DNA polymerase concentration three-fold.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::caf5440862276647c64296cdcf390640 https://doi.org/10.17504/protocols.io.36wgqj9zyvk5/v1 Zobrazit plný text záznamu