Determination of —SH and —SS— groups in proteins. I. A reassessment of the use of methylmercuric iodide

Autor:	C. R. Hamlin, W. F. Forbes
Rok vydání:	1968
Předmět:	Chromatography Chemistry Organic Chemistry General Chemistry Amperometric titration Catalysis Quantitative determination Methylmercuric iodide
Zdroj:	Canadian Journal of Chemistry. 46:3033-3040
ISSN:	1480-3291 0008-4042
Popis:	Amperometric titration with methylmercuric iodide was found to be unsatisfactory for the quantitative determination of —SH and —SS— values in several soluble proteins. It is concluded that the "high reactivity" of the mercurial has previously been overemphasized. Consistent values can, however, be obtained by permitting protein samples to react with an excess of the mercurial, for about 100 h, followed by the polarographic estimation of the remaining reagent. Contrary to general belief, this procedure was found to be more precise than amperometric titration, and, if appropriate precautions are taken, it is applicable to considerably smaller amounts (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::caee7e7d4968f8be59fe4b39448fcceb https://doi.org/10.1139/v68-503 Zobrazit plný text záznamu