Genetically encoded optical activation of DNA recombination in human cells
| Autor: | Alexander Deiters, Rajendra Uprety, Ji Luo, Jason W. Chin, Eyal Arbely, Chungjung Chou, J. Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chemistry HEK 293 cells Metals and Alloys Cre recombinase General Chemistry Integrases Molecular biology Catalysis Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Amino acid Cell biology 03 medical and health sciences 030104 developmental biology law Materials Chemistry Ceramics and Composites Recombinant DNA Recombinase Cre-Lox recombination Floxing |
| Zdroj: | Chemical Communications. 52:8529-8532 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c6cc03934k |
| Popis: | We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination. |
| Databáze: | OpenAIRE |
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