Expression of β-(1,3-1,4)-glucanase gene of Orpinomyces sp. GMLF18 in Escherichia coli EC1000 and Lactococcus lactis subsp. cremoris MG1363
| Autor: | Ferit Can Yazdiç, İsmail Akyol, Emin Özköse, Mehmet Sait Ekinci, Uğur Çömlekcioğlu |
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| Rok vydání: | 2011 |
| Předmět: |
biology
Molecular mass Physiology Lactococcus lactis subsp cremoris Lactococcus lactis Cell Biology Glucanase medicine.disease_cause biology.organism_classification Microbiology law.invention law Hydrolase Genetics medicine Recombinant DNA Orpinomyces General Agricultural and Biological Sciences Molecular Biology Escherichia coli |
| Zdroj: | Turkish Journal of Biology. |
| ISSN: | 1303-6092 |
| DOI: | 10.3906/biy-0912-25 |
| Popis: | A gene encoding b-(1,3-1,4)-glucanase (licA) was amplified from Orpinomyces sp. GMLF18 and expressed in Escherichia coli. The DNA sequence of licA showed that the gene was 707 bp and encoded a protein with a molecular mass of 26 kDa that belongs to family glycosyl hydrolase 16. The main LicA activity was observed to be cell-associated for the licA containing transformant E. coli, and the enzyme expressed in E. coli showed the highest activity at pH 5.0-6.0 and at temperatures of 40-50 °C. The enzyme was found to be stable at 40 °C; however, 12% of LicA activity was lost at 50 °C in 20 min. The licA was then introduced into the facultative anaerobic bacterium Lactococcus lactis subsp. cremoris MG1363 by a stable recombinant plasmid, pIL253. Although the enzymatic activity was lower than that in E. coli, the gene encoding the fungal originated lichenase was successfully expressed in L. lactis. |
| Databáze: | OpenAIRE |
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