Effect ofP15 INK4b/MTS2 on the proliferation of human hepatoma cells SMMC-7721

Autor: Fawu Shi, Hong Zhang, Huitu Liu
Rok vydání: 2000
Předmět:
Zdroj: Chinese Science Bulletin. 45:1408-1412
ISSN: 1861-9541
1001-6538
DOI: 10.1007/bf02886248
Popis: The full length cDNA coding forP15 INK4b, which is a cyclin-dependent kinase inhibitor, was cloned to plasmid PXJ41-neo (Eco R I /Xho I site) and the new constructed plasmid pXJp15 was obtained. pXJp15 was transferred into the human hepatoma SMMC-7721 cetls by lipofectine reagent. After G418 setection, a series of cetl lines stably expressing high levets ofP15 (named SHT) and the clone containing vector PXJ41-neo only (named SVXJ) were obtained by Northern and Western analysis. The results showed that the proliferation of SHT cells is inhibited compared with that of SVXJ cetls. Cell cycle analysis indicated that overexpressing ofP15 inhibited the growth of SHT cetls by decreasing progrssion of cetls from G1 to S and G2 to M phases. The levets ofc-Myc andc-Fos were obviously decreased in SHT cells compared with control cetls by Western blotting. The decreased expression of oncogene may be one of the molecular mechanisms of the effect ofP15 on the proliferation of in SHT cetls.
Databáze: OpenAIRE
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