Phenylbutazone blocks the cytokine response following a high-intensity incremental exercise challenge in horses
| Autor: | N.R. Liburt, Jennifer Streltsova, William C. Franke, Kenneth H. McKeever, A. Betancourt, Robert A. Lehnhard, David W. Horohov, Amanda A. Adams |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Physiology business.industry Veterinary (miscellaneous) Endocrinology Diabetes and Metabolism Biophysics Repeated measures design Horse Placebo Biochemistry Incremental exercise Endocrinology Oral administration Physiology (medical) Internal medicine Anesthesia Phenylbutazone medicine Orthopedics and Sports Medicine Analysis of variance Treadmill business medicine.drug |
| Zdroj: | Comparative Exercise Physiology. 7:103-108 |
| ISSN: | 1755-2559 1755-2540 |
| DOI: | 10.1017/s1755254010000334 |
| Popis: | This study tested the hypothesis that phenylbutazone would block the exercise-induced increase in cytokine markers of inflammation in blood. Blood samples were obtained from unfit Standardbred mares (age 10 ± 4 years, ~500 kg) before and after three different trials (standing control (CON), n = 9; exercise with phenylbutazone (EX-bute), n = 9; and exercise with water, n = 9). Comparisons were made for data collected in three trials, one where each horse underwent an incremental exercise test (graded exercise test (GXT)) where they were administered water as a placebo, a GXT following phenylbutazone administration (2 g given orally 2 h before the GXT) or standing parallel control where they stood quietly in stalls. During the GXT, horses ran on a treadmill (1 m s − 1 increases each min until fatigue, 6% grade). Blood samples were obtained 30 min before exercise, immediately after exercise and at 0.5, 1, 2, 4 and 24 h post-GXT or at matched time points during the parallel control trials. Samples were analysed using real-time PCR for measurement of mRNA expression of interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α) and interleukin (IL)-6 in samples collected during all three trials, and for IL-1 and IL-10 in samples collected for the CON and EX-bute trials. Data were analysed using ANOVA for repeated measures, and where appropriate, post hoc separation of means utilized the Student–Newman–Keuls test. The null hypothesis was rejected when P P >0.05) in IL-1, IL-6, IFN-γ or TNF-α during CON or following phenylbutazone administration. During the water trial, exercise resulted in significant increases in IFN-γ, IL-1 and TNF-α. It was concluded that high-intensity exercise results in a transient increase in the expression of inflammatory cytokines in blood that is blocked when phenylbutazone is administered to horses. |
| Databáze: | OpenAIRE |
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