Activity of Bacillus thuringiensis hybrid protein against a lepidopteran and a coleopteran pest

Autor:	Sonia Ospina, Silvio Alejandro López-Pazos, Jairo Cerón, Adriana Carolina Rojas Arias
Rok vydání:	2010
Předmět:	biology Toxin fungi Biological pest control Tecia solanivora Gelechiidae biology.organism_classification medicine.disease_cause Microbiology Lepidoptera genitalia Bacillus thuringiensis Botany Genetics medicine Instar PEST analysis Molecular Biology
Zdroj:	FEMS Microbiology Letters. 302:93-98
ISSN:	1574-6968 0378-1097
Popis:	The use of Cry proteins from Bacillus thuringiensis are an important strategy for biological control. Recently it has been demonstrated that Cry hybrid proteins (by domain swapping) resulted in improved toxicities in comparison with parental proteins. Here, an SN1917 hybrid toxin was constructed and tested against Colombian pest insects Tecia solanivora (Lepidoptera: Gelechiidae), a severe potato pest, and Hypothenemus hampei (Coleoptera: Scolytidae), which attacks coffee crops. The SN1917 protoxin had a concentration causing 50% mortality (LC50) of 392 ng cm–2, and SN1917 toxin showed an LC50 of 201 ng cm–2 against T. solanivora first instar larvae. The two parental toxins (Cry1B and Cry1I) used to make this new hybrid protein caused a mortality of 60% and 52%, respectively. Unfortunately, H. hampei first instar larvae proved to be resistant to the toxin. We conclude that SN1917 is an option for biological control and resistance management of T. solanivora. Implications for H. hampei are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::caaf237276864a7c1b2ff7e6d2ee2c3e https://doi.org/10.1111/j.1574-6968.2009.01821.x Zobrazit plný text záznamu Plný text