Identification of a Genetic Marker That Discriminates Ocean-Type and Stream-Type Chinook Salmon in the Columbia River Basin

Autor:	Russell J. Rodriguez, David R. Clifton, James L. Holloway, Carl O. Ostberg, Charlotte Rasmussen
Rok vydání:	2003
Předmět:	geography Chinook wind geography.geographical_feature_category Ecology Drainage basin Aquatic Science Biology biology.organism_classification Hatchery RAPD Genetic marker Threatened species Oncorhynchus Ecology Evolution Behavior and Systematics Salmonidae
Zdroj:	Transactions of the American Fisheries Society. 132:131-142
ISSN:	1548-8659 0002-8487
DOI:	10.1577/1548-8659(2003)132<0131:ioagmt>2.0.co;2
Popis:	A marker based on randomly amplified polymorphic DNA (RAPD), OT-38, was discovered that nonlethally discriminates between stream-type and ocean-type populations of chinook salmon Oncorhynchus tshawytscha in the Columbia River basin, including the threatened fall-run (ocean-type) and spring-run (stream-type) Snake River populations. This marker was developed by amplifying chinook salmon genomic DNA with a single RAPD primer, sequencing the termini of the polymorphic products, and designing primer pairs for allele-specific amplification. It was used to assay 18−80 individuals from several wild and hatchery populations differing in year-class, freshwater life history, and location along the Columbia River. OT-38 unambiguously distinguished ocean-type from stream-type populations in 93.1% of the chinook salmon sampled.
Databáze:	OpenAIRE
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