| Autor: |
Norman Barnabé, Michael Butler, Martin Berry, D. Moffatt, Megan Patrick, N. Huzel, A. Burgener, Kevin M. Coombs |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Biotechnology Progress. 16:854-858 |
| ISSN: |
8756-7938 |
| DOI: |
10.1021/bp000110+ |
| Popis: |
Two strains of reovirus (serotype 1 Lang/TIL and serotype 3 Dearing/T3D) were propagated in Vero cells grown in stationary or agitated cultures in a serum-free medium, M-VSFM. Solid microcarriers (Cytodex-1) were used to support cell growth in agitated cultures with a normal doubling time of 25 h. Cell yields of 1 x 10(6) cells/mL were obtained from an inoculum of 2 x 10(5) cells/mL in 4 days in microcarrier cultures. The growth profile and cell yield was not significantly different from serum-supplemented cultures. The virus titer increased by 3-4 orders of magnitude over a culture period of 150 h. The maximum virus titer in stationary cultures reached >1 x 10(9) pfu/mL for both strains of reovirus in M-VSFM. M-VSFM also supported high viral yields in microcarrier cultures. Both the specific productivity and final viral yield was higher in M-VSFM than serum-supplemented cultures. The high viral productivity suggests that this is a suitable system for the production of reovirus as an oncolytic agent for human therapeutic use. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Plný text