Interaction of toremifene with dipalmitoyl-phosphatidyl-glycerol in monolayers at the air–water interface followed by fluorescence microscopy in Langmuir–Blodgett films
| Autor: | Amélia M.P.S. Gonçalves da Silva, José M. G. Martinho, Rute I. S. Romão, Ermelinda M. S. Maçôas |
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| Rok vydání: | 2013 |
| Předmět: |
Langmuir
Brewster's angle Chemistry Metals and Alloys Analytical chemistry Protonation Surfaces and Interfaces Mole fraction Langmuir–Blodgett film Surfaces Coatings and Films Electronic Optical and Magnetic Materials Rhodamine symbols.namesake Crystallography chemistry.chemical_compound Monolayer Materials Chemistry symbols Fluorescence microscope |
| Zdroj: | Thin Solid Films. 534:584-590 |
| ISSN: | 0040-6090 |
| DOI: | 10.1016/j.tsf.2013.02.052 |
| Popis: | Langmuir monolayers of dipalmitoyl-phosphatidyl-glycerol (DPPG) containing toremifene (TOR), an antiestrogen drug derivative of tamoxifen, were prepared in order to study the interaction of the drug with the cell membrane. TOR is not surface active but it remains at the interface in DPPG rich monolayers anchored by electrostatic interaction with the anionic DPPG up to the equimolar composition. The fluidity of mixed monolayers increases up to the TOR mole fraction X TOR = 0.3, remaining practically invariant for higher compositions. Brewster angle microscopy shows that the TOR disturbs the DPPG organization and the liquid condensed (LC) domains of DPPG become undetectable for X TOR ≥ 0.4. Laser scanning confocal fluorescence microscopy images of the LB films doped with rhodamine B-piperazine amide dye confirm the progressive reduction in size of LC domains, from which TOR and rhodamine are excluded. The incorporation of TOR in DPPG monolayers up to the equimolar composition supports the formation of a TOR:DPPG complex (1:1) due to electrostatic interactions between the negatively charged polar groups of DPPG and protonated TOR. |
| Databáze: | OpenAIRE |
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