First Evidence of DAAM1 Localization During the Post-Natal Development of Rat Testis and in Mammalian Sperm
Autor: | Massimo Venditti, Francesco Aniello, Raffaele Dotolo, Aldo Donizetti, Sergio Minucci, Paolo Pariante, Diana Ferrara |
---|---|
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
DAAM1 030219 obstetrics & reproductive medicine Physiology Clinical Biochemistry Morphogenesis Cell Biology Biology Sertoli cell Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Gonocyte Cell polarity medicine Gamete Cytoskeleton Germ cell |
Zdroj: | Journal of Cellular Physiology. 231:2172-2184 |
ISSN: | 0021-9541 |
DOI: | 10.1002/jcp.25330 |
Popis: | Dishevelled-associated activator of morphogenesis 1 (DAAM1) is a formin-family protein involved in nucleation of unbranched actin filaments and in cytoskeletal organization through Wnt-Dishevelled PCP pathway, which participates in essential biological processes, such as cell polarity, movement, and adhesion during morphogenesis and organogenesis. While its role has been investigated during development and in somatic cells, its potential association with the germinal compartment and reproduction is still unexplored. In this work, we assessed the possible association of DAAM1 with the morphogenesis of rat testis. We studied its expression and profiled its localization versus actin and tubulin, during the first wave of spermatogenesis and in the adult gonad (from 7 to 60 dpp). We show that, in mitotic phases, DAAM1 shares its localization with actin in Sertoli cells, gonocytes, and spermatogonia. Later, during meiosis, both proteins are found in spermatocytes, while only actin is detectable at the forming blood-testis barrier. DAAM1, then, follows the development of the acrosome system throughout spermiogenesis, and it is finally retained inside the cytoplasmic droplet in mature gametes, as corroborated by additional immunolocalization data on both rat and human sperm. Unlike the DAAM1, actin keeps its localization in Sertoli cells, and tubulin is associated with their protruding cytoplasm during the process. Our data support, for the first time, the hypothesis of a role for DAAM1 in cytoskeletal organization during Mammalian testis morphogenesis and gamete progression, while also hinting at its possible investigation as a morphological marker of germ cell and sperm physiology. J. Cell. Physiol. 231: 2172-2184, 2016. © 2016 Wiley Periodicals, Inc. |
Databáze: | OpenAIRE |
Externí odkaz: |