Expression of CCR8 is increased in asthma
| Autor: | Ian D. Pavord, C Raport, Roland Kolbeck, Andrew J. Wardlaw, Christopher E. Brightling, Dulce Soler, K Mutalithas, C. Guillen |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Chemokine CD3 Immunology CCL1 CCR8 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Immunology and Allergy Respiratory system 030304 developmental biology 0303 health sciences Lung medicine.diagnostic_test biology business.industry T lymphocyte respiratory system respiratory tract diseases 3. Good health Endocrinology Bronchoalveolar lavage medicine.anatomical_structure biology.protein business 030215 immunology |
| Zdroj: | Clinical & Experimental Allergy. 40:1175-1185 |
| ISSN: | 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2010.03504.x |
| Popis: | BACKGROUND: Chemokines and their receptors could play key roles in the recruitment of T cells to the asthmatic lung. CCR8 is preferentially expressed on T-helper type 2 cells, and is thought to play a role in the pathogenesis of human asthma. OBJECTIVE: Determine the expression of CCR8 on T cells in blood, bronchoalveolar lavage (BAL) and bronchial mucosa from asthmatics and normal subjects. METHODS: CCR8 expression in blood and BAL from asthma and normal subjects was studied using flow cytometry. CCR8 expression on IFN-gamma+ and IL-4+/IL-13+ blood and BAL T cells was studied following stimulation with Phorbol-Myristate-Acetate and Calcium Ionophore. Paraffin-embedded bronchial biopsies were used to study CCR8 in bronchial epithelium. RESULTS: The percentage of CD3+ cells expressing CCR8 in the blood was higher in asthmatics (4.7+/-0.4%) compared with normal subjects (3.0+/-0.4%; P |
| Databáze: | OpenAIRE |
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