An improvedAgrobacterium-mediated transformation protocol for recalcitrant elite indica rice cultivars

Autor:	Duraialagaraja Sudhakar, P. Balasubramanian, S. Maruthasalam, K. K. Kumar, M. Loganathan
Rok vydání:	2005
Předmět:	Reporter gene Agrobacterium Transgene food and beverages Plant Science Biology biology.organism_classification Genetically modified rice Transformation (genetics) Botany Cultivar Molecular Biology Selectable marker Transformation efficiency
Zdroj:	Plant Molecular Biology Reporter. 23:67-73
ISSN:	1572-9818 0735-9640
Popis:	We report here a high-efficiency transformation protocol for recalcitrant indica rice cultivars IR64 and IR72 with the selectable marker genehph and thegusA reporter gene. Factors that favor high-efficiency transformation were found to be use of 2-month-old mature seed-derived embryogenic calli, maltose as a source of carbon, a higher concentration of 2,4-dichlorophenoxyacetic acid, and both phytagel and agar as gelling agents. The putative transgenic (T0) plants were analyzed for integration of the transgene through polymerase chain reaction and Southern blotting analyses. Various factors thought to be responsible for increased transformation efficiency are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ca5e492341151c9a6a8a43329e309493 https://doi.org/10.1007/bf02772648 Zobrazit plný text záznamu Plný text ve formátu PDF