Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model
| Autor: | Hussam Al Hussein, Marius Harpa, Ionela Movileanu, Hamida Al Hussein, Horatiu Suciu, Klara Branzaniuc, Dan Simionescu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty Isolation (health care) business.industry Materials Science (miscellaneous) Process Chemistry and Technology General Engineering Adipose tissue General Chemistry General Medicine General Biochemistry Genetics and Molecular Biology Materials Chemistry medicine General Pharmacology Toxicology and Pharmaceutics business |
| Zdroj: | Revista de Chimie. 70:1826-1828 |
| ISSN: | 2668-8212 0034-7752 |
| DOI: | 10.37358/rc.19.5.7224 |
| Popis: | Many methods of stem cells collection and isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering. The present study purposed to describe a minimally invasive surgical protocol for adipose tissue collection from sheep�s inter-scapular area. The procedure was carried out on adult sheeps, in aseptic conditions. A light sedation protocol with Detomidine was performed, the recovery from anesthesia being carried out with Atipamezole. Throughout the sedation, the surgical procedure and the recovery from anesthesia, the vital functions of the animal were monitored. The adipose tissue samples collected in sterile tubes with culture medium (Dulbecco�s modified Eagle�s medium - DMEM/10% / FBS10 - fetal bovine serum, 2% antibiotic/antifungal), have been succesfully used by our research team for adipose tissue derived stem cells (ADSCs) isolation for further use in cardiac valves tissue engineering. |
| Databáze: | OpenAIRE |
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