Fluorescein-Based ‘‘Turn On’’ Fluorescence Detection of Zn2+and Its Applications in Imaging of Zn2+in Apoptotic Cells
| Autor: | Remani Vasudevan Sumesh, Gandhi Sivaraman, Duraisamy Chellappa, Balasubramanian Vidya |
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| Rok vydání: | 2016 |
| Předmět: |
inorganic chemicals
biology 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry biology.organism_classification 01 natural sciences Fluorescence 0104 chemical sciences Ion Absorbance HeLa chemistry.chemical_compound chemistry Live cell imaging biological sciences Imidazole Titration Fluorescein 0210 nano-technology |
| Zdroj: | ChemistrySelect. 1:4024-4029 |
| ISSN: | 2365-6549 |
| DOI: | 10.1002/slct.201600863 |
| Popis: | Fluorescein dye bearing imidazole has been designed and synthesized as an effective colorimetric and ‘‘turn on’’ fluorescent sensor for Zn2+. The probe FI-1 shows selective and dramatic colour change from colourless to yellow and fluorescence turn-on response for Zn2+ with greater sensitivity. 1H NMR titration experiments confirms that the binding of Zn2+ ions leads to the spirolactam ring opening that causes change in absorbance and fluorescence. Density functional theory calculations also support the observed photophysical changes. The applicability of the probe for Zn2+ detection was shown by the live cell imaging in HeLa cells. Further the probe utilized for imaging of Zn2+ ions liberated during apoptosis. |
| Databáze: | OpenAIRE |
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