Freeze-fracture electron microscopy and enzyme activity of reconstituted cytochrome oxidase

Autor:	Peter Nicholls, M. Tihova, Brenda Tattrie
Rok vydání:	1992
Předmět:	biology Chemistry law Fracture (mineralogy) Biophysics biology.protein Cytochrome c oxidase General Medicine Electron microscope Enzyme assay law.invention
Zdroj:	Proceedings, annual meeting, Electron Microscopy Society of America. 50:710-711
ISSN:	2690-1315 0424-8201
DOI:	10.1017/s0424820100123957
Popis:	Eukaryotic cytochrome oxidase (CO) is a multisubunit enzyme spanning the inner mitochondrial membrane. As the terminal component in the respiratory assembly it catalyzes the reduction of oxygen to water. The size, shape and other gross features of the 3-dimensional structure of this protein have been obtained only for its 2-dimensional crystal form. The enzyme can be incorporated into liposomes of a defined lipid composition. Freeze-fracture electron microscopy of cytochrome oxidase vesicles (COV) has been combined with kinetic and spectroscopic measurements to study the phospholipid requirements for enzyme activity and protein orientation within the bilayers.
Databáze:	OpenAIRE
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