STABILITY-INDICATING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF ANTIHISTAMINE DRUG AZELASTINE
| Autor: | Pasha Ty, Shital Patel |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Pharmacology
Detection limit Chromatography Correlation coefficient Azelastine Hydrochloride Chemistry Potassium Pharmaceutical Science chemistry.chemical_element High-performance liquid chromatography Azelastine chemistry.chemical_compound medicine Pharmacology (medical) Particle size Acetonitrile medicine.drug |
| Zdroj: | Asian Journal of Pharmaceutical and Clinical Research. 11:248 |
| ISSN: | 2455-3891 0974-2441 |
| DOI: | 10.22159/ajpcr.2018.v11i8.25506 |
| Popis: | Objective: The objective of this research was to develop a simple, precise, accurate, and stability-indicating reverse-phase high-performance liquid chromatographic method for estimation of azelastine hydrochloride (AZL) in nasal spray preparation.Methods: Chromatography was performed on a 250 mm×4.6 mm, 5-μm particle size, Waters Spherisorb CN column using (50:50 v/v) mixture of potassium dihydrogen phosphate buffer and acetonitrile as mobile phase. The detection was carried out at 290 nm and flow rate employed was 1.0 ml/min. The degradation of AZL was studied under different ICH recommended stress conditions.Results: The retention time was 4.34 min for AZL. Linearity was established in the concentration range of 5–120 μg/ml, with a correlation coefficient of 0.9996. Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.81 μg/ml and 2.44 μg/ml, respectively. Percentage recovery was found between 99 and 102%. The values of percentage relative standard deviation ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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