Popis: |
Advances in knowledge about the molecular structure of plant genome and development of techniques to rapidly characterise genomic variations have established DNA fingerprinting as a powerful tool for identifying plant species, varieties, clones, individuals and even plant products. This article provides details of DNA polymorphism in plants and classical and more recent molecular marker techniques based on restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR), DNA microarrays and sequencing. The advantages and limitations of each technique are given along with some recent examples of their application in plant identification. |