Influence of Insertion of the Last Sense Codon on Expression Efficiency of Green Fluorescent Protein Gene in Escherichia coli

Autor:	Shota Inoue, Xiaoliang Hao, Masahide Ishikawa
Rok vydání:	2015
Předmět:	Sense Codon Fluorescence intensity Materials science Codon usage bias Gene expression medicine medicine.disease_cause Gene Escherichia coli Molecular biology Stop codon Green fluorescent protein
Zdroj:	Journal of Materials Science and Chemical Engineering. :13-18
ISSN:	2327-6053 2327-6045
DOI:	10.4236/msce.2015.36003
Popis:	We studied the relationship between insertion of the last sense codon (i.e., the codon preceding the stop codon) and the efficiency of gene expression. We inserted 64 kinds of last sense codon at the 5’ end of the stop codon of the green fluorescent protein (GFP) gene and introduced the modified GFP genes into Escherichia coli (E. coli). Measuring the fluorescence intensity of the GFP produced in E. coli showed that the last sense codon influenced GFP gene expression and when CCG was inserted as the last sense codon, fluorescence intensity of E. coli was increased to 2.09 fold. On the other hand, insertion of CUA caused decrease of fluorescence intensity to 0.33 fold. We hope that our findings, which may be applicable to gene engineering, will be useful for further studies of protein expression.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ca07d2a434f15d6ba14ac9590d2b0f88 https://doi.org/10.4236/msce.2015.36003 Zobrazit plný text záznamu