Synthesis of the β-amyloid fragment 5RHDSGY10 and its isomers

Autor:	E. Yu. Aleshina, E. F. Kolesanova, Oleg N. Kharybin, M. A. Sanzhakov, A. A. Moisa, N. V. Pyndyk, Eugene N. Nikolaev
Rok vydání:	2008
Předmět:	chemistry.chemical_classification Chemistry Stereochemistry Clinical Biochemistry Peptide Biochemistry Mass spectrometric High-performance liquid chromatography Combinatorial chemistry β amyloid Yield (chemistry) Reagent Molecular Medicine Bioorganic chemistry
Zdroj:	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 2:288-292
ISSN:	1990-7516 1990-7508
Popis:	The peptide RHDSGY, a fragment of the human β-amyloid Zn-binding site, and its isomers RH(D-Asp)SGY and RH(β-Asp)SGY have been obtained as amides by means of solid-phase synthesis and analyzed by HPLC and various mass spectrometric methods. The problem of low yield of the RHDSGY peptide and its isomers attributed to 9-fluorenylmethoxycarbonyl (Fmoc)-amino acids and/or formation of such side-products as RH(β-Asp)SGY (or RHDSGY during synthesis of RH(β-Asp)SGY) and RH(Asp-imide) SGY was solved via selection of individual reagents for removal of Fmoc groups from α-amino groups of the growing peptide chain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ca006324a87c448cdeb6c003b9253f77 https://doi.org/10.1134/s1990750808030098 Zobrazit plný text záznamu Full text from SpringerLink