Biphasic influence ofStaphylococcus aureuson human epidermal tight junctions
| Autor: | Hélène Duplan, Pia Houdek, Germar Schüring, Katja Bäsler, Béatrice Guiraud, Sabine Vidal-y-Sy, Marie-Florence Galliano, Stefan W. Schneider, Thomas Volksdorf, E Wladykowski, Antony Caruana, Holger Rohde, Sophia Bergmann, Johanna M. Brandner, Sandrine Bessou-Touya |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Tight junction General Neuroscience Biology Occludin medicine.disease_cause General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine Microbiology 03 medical and health sciences HaCaT 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure History and Philosophy of Science Staphylococcus aureus 030220 oncology & carcinogenesis medicine Claudin Keratinocyte Barrier function |
| Zdroj: | Annals of the New York Academy of Sciences. 1405:53-70 |
| ISSN: | 0077-8923 |
| Popis: | Bacterial infections (e.g., with Staphylococcus aureus) are serious problems in skin with a compromised barrier, such as in patients with atopic dermatitis. Previously, it was shown that tight junction (TJ) proteins are influenced by staphylococcal infection, and TJ function is impaired after infection of the keratinocyte cell line HaCaT. However, functional studies in cells or models more similar to human skin are missing. Therefore, we investigated bacterial colonialization and infection with live S. aureus in primary human keratinocytes and reconstructed human epidermis (RHE). We show that short-term inoculation results in increased TJ barrier function-which could not be seen in HaCaT cells-hinting at an early protective effect. This is accompanied by occludin phosphorylation and sustained localization of occludin and claudin-4 at cell membranes. Long-term incubation resulted in decreased presence of claudin-1 and claudin-4 at cell membranes and decreased TJ barrier function. The agr regulon of S. aureus plays a role in the increasing but not in the decreasing effect. Proinflammatory cytokines, which are produced as a result of S. aureus inoculation, influence both phases. In summary, we show here that S. aureus can have short-term promoting effects on the TJ barrier, while in the long term it results in disturbance of TJs. |
| Databáze: | OpenAIRE |
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