Characterization of autoantigens and autoantibodies by immunoblotting

Autor: Andrew P. Parnassa, Jia Li‐Chu, Keith B. Elkon, Eloisa Bonfa
Rok vydání: 1987
Předmět:
Zdroj: Electrophoresis. 8:445-451
ISSN: 1522-2683
0173-0835
DOI: 10.1002/elps.1150080914
Popis: Immunoblotting has proven to be one of the most useful methods for detection of antibodies and partial characterization of proteins that are antigenic in the autoimmune diseases. The basic physicochemical characteristics (size and charge) of most of the major autoantigens have now been described. By combining various electrophoretic techniques with immunoblotting, a number of new applications have been reported. Two-dimensional electrophoresis was used to fingerprint autoantigens and detect post-translational modifications. Composite gel electrophoresis resolved ribonucleprotein complexes and was also used to analyze IgA, IgM autoantibodies and to determine the composition of cryoglobulins. Autoantibody heterogeneity was analyzed by isoelectric focusing. In some studies, the subclass, idiotype, or antigen binding specificity of autoantibodies could be determined by immunoblotting procedures. Coupled with improvements in antigen purity (synthetic peptides and recombinant proteins) and wider availability of monospecific antibodies, immunoblotting will continue to be a versatile tool in basic and clinical research in autoimmunity.
Databáze: OpenAIRE
Externí odkaz: