Popis: |
The growth of the gill, ventricle, red muscle, and white muscle in terms of wet weight and total protein content was measured in trout (Salmo gairdneri; wt 20-370 g) at 12 C. In vivo protein synthesis rates were measured following a single high dose of ³H-phenylalanine, which stabilized specific radioactivity in the free pools. Protein degradation rates were calculated from the difference between synthesis and net protein accretion. During growth fractional synthesis and degradation rates fell in all the tissues. Comparisons between the four tissue types showed that fractional degradation rates were directly correlated with fractional synthesis rates. Throughout growth the tissue types showed the same ranking in fractional synthesis and degradation rates, i.e., gills > ventricle > red muscle > white muscle. Calculation of the total amounts of protein synthesized per day revealed that the tissue types were ranked in the following way: white muscle = gills > red muscle > ventricle. Seventy-six percent of th... |