Comparative Study of the DNA-Damaging Activity of Epichlorohydrin Using Escherichia coli Biosensors and the Comet Assay Method in Mice

Autor:	B. S. Zhoshibekova, K. G. Ordzhonikidze, E. V. Igonina, S. K. Abilev
Rok vydání:	2021
Předmět:	DNA damage organic chemicals fungi Biology medicine.disease_cause Molecular biology Comet assay chemistry.chemical_compound chemistry In vivo Genetics medicine Epichlorohydrin SOS response Escherichia coli Gene DNA
Zdroj:	Russian Journal of Genetics. 57:1035-1041
ISSN:	1608-3369 1022-7954
DOI:	10.1134/s1022795421090088
Popis:	A comparative study of the genotoxic effects of methylmethanesulfonate (MMS) and epichlorohydrin (ECH) was performed using bacterial E. coli biosensors and the comet assay method in mice. ECH was shown to induce weaker SOS response and expression of the alkA gene in bacterial cells compared to MMS. In vivo experiments on mice using the comet assay method showed DNA-damaging activity of ECH in cells of the liver, kidneys, and lungs after 3 h of exposure, same as of MMS. The levels of DNA damage caused by ECH in these organs after exposure for 3 h were lower than for MMS. The genotoxic effect of ECH after 18 h of exposure was statistically significant only in a dose of 20 mg/kg in kidney cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c9e44385cf5819782dc6959e8475347f https://doi.org/10.1134/s1022795421090088 Zobrazit plný text záznamu Full text from SpringerLink