The amino acid at position 624 in the glycoprotein of SFTSV (severe fever with thrombocytopenia virus) plays a critical role in low-pH-dependent cell fusion activity

Autor:	Kumiko Yoshimatsu, Sanae Nishio, Manabu Igarashi, Kenta Shimizu, Ryo Ito, Yoshimi Tsuda, Jiro Arikawa
Rok vydání:	2017
Předmět:	0301 basic medicine chemistry.chemical_classification Cell fusion Arginine General Medicine Virology General Biochemistry Genetics and Molecular Biology Virus Amino acid Serine 03 medical and health sciences 030104 developmental biology chemistry Glycine Aspartic acid Peptide sequence
Zdroj:	Biomedical Research. 38:89-97
ISSN:	1880-313X 0388-6107
Popis:	Severe fever with thrombocytopenia syndrome virus (SFTSV) is a novel phlebovirus responsible for causing an emerging zoonotic disease. We previously established subclones from SFTSV strain YG1 based on differences in low-pH-dependent cell fusion activities and found two amino acid substitutions, Y328H and R624W, in the envelope glycoprotein (GP) of high fusion subclones. In this study, we show that transiently expressed GP with the R624W mutation, but not the Y328H mutation, induced cell fusion under acidic conditions. GP possessing either tryptophan, serine, glycine or aspartic acid at position 624 induced cell fusion, whereas GP possessing basic amino acids such as arginine or lysine did not induce cell fusion. These results indicated that the amino acid at position 624 has an important role for inducing low-pH-dependent cell fusion.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c9d865e279d4aac534a4650cc6e1fe6a https://doi.org/10.2220/biomedres.38.89 Zobrazit plný text záznamu