| Autor: |
Richard B. Mortensen, Lynn M. Kochersperger, Lois A. Aldwin, Cynthia L. Adams, Christopher P. Holmes |
| Rok vydání: |
1994 |
| Předmět: |
|
| DOI: |
10.1016/b978-0-12-194710-1.50064-3 |
| Popis: |
Publisher Summary This chapter describes new methodologies in the preparation of peptide libraries. The photochemical synthesis of peptides is facilitated through the use of nitroveratryloxycarbonyl (Nvoc) as the protecting group for the terminal amine of the growing peptide chain. The Nvoc-derivatized amines are unreactive until deprotected by light, and irradiation with 365 nm UV light causes it to undergo cleavage to a nitrosoaldehyde, carbon dioxide, and the liberated free amino group. The presence of a mild acid in the photolysis solvent renders the liberated amine unreactive toward the photo byproducts, and quantitative yields of amines can be obtained. The second step of the process is the exposure of the surface to a solution of a carboxyl-activated Nvoc-protected amino acid. Washing of the surface to remove excess free amino acid with a variety of solvents renders the surface ready for the next photodeprotection step. The entire process can be made spatially addressable by irradiating through lithographic masks. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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