Comparison of neutrophil respiratory oxidative burst activity between flow cytometry using dihydrorhodamine (DHR) 123 and conventional nitroblue tetrazolium test (NBT)
| Autor: | Nurasyikin Yusof, Norafiza Mohd Yasin, Rabeya Yousuf, Asrul Abdul Wahab, Suria Abdul Aziz |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Bangladesh Journal of Medical Science. 21:626-633 |
| ISSN: | 2076-0299 2223-4721 |
| DOI: | 10.3329/bjms.v21i3.59577 |
| Popis: | Background: Neutrophil plays a defense role against bacteria and fungi specifically by the neutrophil phagoburst activity. The nitroblue tetrazolium (NBT) test is the gold standard method to measure this but the flow cytometry assays also have been developed. The aim of the study was to evaluate the performance of flow cytometry using Dihydrorhodamine (DHR) 123 and to compare with the conventional NBT test. Methods: This study was carried out to determine the phagoburst activity at Universiti Kebangsaan Malaysia Medical Centre on 26 normal healthy donors and 5 chronic myeloid leukemia (CML) patients using both methods. Results: In flow cytometry, higher fluorescence intensity was observed in Phorbol myristate acetate (PMA) stimulated neutrophils. Comparing the mean fluorescent intensity (MFI) between CML and healthy donor showed CML patients have significant lower MFI (312.15 vs 738.22; p95% of stimulated cells suggest the higher sensitivity. All samples with negative NBT showed negative DHR, reflecting high degree of agreement between these two methods and eliminating possible false negative result in flow cytometry. Conclusion: This study showed that flow cytometry assay has superior technical advantages compared to NBT test as it is easy to perform, quick, more sensitive and requires only a small amount of blood sample. Bangladesh Journal of Medical Science Vol. 21 No. 03 July’22 Page: 626-633 |
| Databáze: | OpenAIRE |
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