Use of Experimental Factorial Design for Optimization of Hexavalent Chromium Removal by a Bacterial Consortium: Soil Microcosm Bioremediation

Autor: Wifak Bahafid, Hanane Sayel, Nezha Tahri Joutey, Faouzi Errachidi, Hajar Maâtaoui, Naïma El Ghachtouli
Rok vydání: 2015
Předmět:
Zdroj: Soil and Sediment Contamination: An International Journal. 24:129-142
ISSN: 1549-7887
1532-0383
DOI: 10.1080/15320383.2014.922931
Popis: Hexavalent chromium Cr(VI) is regularly introduced into the environment through diverse anthropogenic activities. It is highly toxic, mutagenic and carcinogenic, and because of its solubility in water, chromate contamination can be difficult to contain. Bacteria can reduce chromate to insoluble and less toxic trivalent chromium Cr(III), and thus increasing attention is paid to chromate bioremediation to reduce its ecotoxicological impacts. In this study, the factorial design 23 was employed to optimize critical parameters responsible for higher Cr(VI) removal by a bacterial consortium. The factors considered were pH, temperature, and inoculum size at two markedly different levels. All three dependent variables have significant effect on Cr(VI) reduction. Optimal Cr(VI) removal by the bacterial consortium occurred at pH 9, temperature 37°C, and inoculum size OD = 3. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.984, thus ensuring a satisfactory adjustment of the se...
Databáze: OpenAIRE
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