| Autor: |
E. A. Zelepuga, B. M. Kolikov, N. B. Katushkina |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Applied Biochemistry and Microbiology. 40:22-24 |
| ISSN: |
0003-6838 |
| DOI: |
10.1023/b:abim.0000010345.70519.23 |
| Popis: |
A method for separation of albumin–ribonuclease (RNase) conjugates has been proposed based on the use of macroporous silicates. It was established that about 76% of ligand-free human serum albumin (LFHSA) formed complexes with enzymes. It was shown that most of the conjugates of albumin and pancreatic RNase contained up to 2 mol enzyme per 1 mol LFHSA. The conjugates of albumin and bacterial RNase isolated from cells of the strain Bacillus intermedius 7P displayed higher specific activities, containing, on average, 2.3 mol RNase per 1 mol LFHSA (for the conjugates with molecular weights below 92 kDa) or 3.3 mol RNase per 1 mol protein carrier (for the conjugates with higher molecular weight). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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