Production of cytokines by alveolar and peritoneal macrophages stimulated byAspergillus fumigatusconidia or hyphae
| Autor: | Donatella Taramelli, Maria Grazia Malabarba, G. Cocuzza, G. Sala, Nicoletta Basilico |
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| Rok vydání: | 1996 |
| Předmět: |
Aspergillus
Cellular immunity biology Hypha medicine.medical_treatment fungi General Medicine biology.organism_classification Aspergillus fumigatus Microbiology Infectious Diseases Cytokine medicine.anatomical_structure medicine Macrophage Tumor necrosis factor alpha Pulmonary alveolus skin and connective tissue diseases |
| Zdroj: | Medical Mycology. 34:49-56 |
| ISSN: | 1460-2709 1369-3786 |
| DOI: | 10.1080/02681219680000081 |
| Popis: | Aspergillus fumigatus is an opportunistic pathogen responsible for severe, invasive infections in neutropenic hosts. Lung clearance of A. fumigatus conidia seems to be mediated by phagocytic cells and oxygen radicals. It is not known if cytokines or nitrogen radicals are also involved. We tested for the production of TNFα, IL-1 and nitric oxide (NO) after stimulation of mouse macrophages with the fungus. We found that both cytokines, but not NO, were produced in a dose-dependent manner during the first 24h of culture. Except for a faster kinetic, no appreciable differences were seen between alveolar and peritoneal macrophages. Furthermore, both hyphae and conidia, either alive or killed, were capable of inducing cytokines production. However, among different Aspergillus spp. only A. fumigatus and A. flavus seemed to induce significant amount of TNFα and IL-1, whereas A. terreus and A. niger were less effective. In no case could we detect production of NO. Finally, macrophages from dexamethasone-treated mi... |
| Databáze: | OpenAIRE |
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