Strain-specific susceptibility towards ventilator-induced lung injury in infant rats
| Autor: | Jörg Ersch, Francesco Greco, Susanne Wiegert, Cannizzaro Vincenzo |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Lung medicine.diagnostic_test business.industry Hysteresivity respiratory system Lung injury respiratory tract diseases Bronchoalveolar lavage Endocrinology Airway resistance medicine.anatomical_structure Internal medicine medicine Breathing Lung volumes business Tidal volume |
| Zdroj: | 7.5 Neonatology and Paediatric Intensive Care. |
| DOI: | 10.1183/13993003.congress-2015.pa1855 |
| Popis: | The importance of strain selection in infant rat models investigating paediatric ventilator-induced lung injury is underestimated. We designed this study to examine the impact of ventilation strategies on lung function and inflammation of two commonly used rat strains, i.e. Sprague Dawley (SD) and Wistar (W) rats. We hypothesised that strain-related differences are physiologically relevant. Two week old rats were allocated to the following strategies: a) low stretch: tidal volume (VT) of 7 mL/kg and positive end-expiratory pressure (PEEP) of 6 cmH2O; b) high stretch: VT of 21 mL/kg and PEEP of 2 cmH2O. Rats were ventilated for 8 h and airway resistance (Raw) and the coefficients of tissue elastance (H) and damping (G) were obtained every 2 h from low-frequency forced oscillatory impedance. Bronchoalveolar lavage fluid (BALF) was obtained for analyses of inflammatory biomarkers. SD rats showed significantly higher Raw and H values after high stretch ventilation when compared to W rats. In contrast, G and hysteresivity (η) significantly increased after high stretch ventilation in W rats only. IL-6 concentrations in BALF did not differ between strategies, whereas MIP-2 was significantly higher in SD rats ventilated with high stretch. Also, albumin concentrations in BALF did not differ between strategies. Our results are not in line with experimental adult rodent data and underscore the relevance of strain and age selection. In addition, changes in respiratory system mechanics can be explained by lung volume changes in SD rats, while the increase in η observed in W rats might reflect structural lung changes. |
| Databáze: | OpenAIRE |
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