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Enhanced antigen–antibody interactions in microfluidic immunosensors can be effected by tailoring the surface free energies of the antibody immobilized surfaces to obtain the appropriate fluid–wall interactions. We report a systematic study to evaluate the surface free energies from contact angle measurements, using the LWAB method, of different antibody (anti-BSA, anti-PSA, and anti-CRP) surfaces, each immobilized separately on to non- and nanotextured Si surfaces via a stack of functionalized layers including aminosilanes of which three different types were used. The apolar surface free energy components were independent of the physical modification in the non-functionalized and the intermediate hydrolyzed surfaces where as they depended on the nature of the surface and the chemical modifications in the subsequent functionalized stages. Surface free energies of the different antibodies immobilized with the shorter chain length aminosilane (APTES) on non- and nanotextured surfaces were in the order of anti-BSA |
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