Fluorescent black phosphorus quantum dots as label-free sensing probes for evaluation of acetylcholinesterase activity
| Autor: | Yuezhong Xian, Caiping Ding, Cuiling Zhang, Xueyu Pei, Wei Gu, Yinghan Yan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
DTNB Metals and Alloys Analytical chemistry Substrate (chemistry) Quantum yield 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Quantum dot Reagent Acetylthiocholine Materials Chemistry Thiol Electrical and Electronic Engineering 0210 nano-technology Instrumentation Nuclear chemistry |
| Zdroj: | Sensors and Actuators B: Chemical. 250:601-607 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2017.05.017 |
| Popis: | In this work, a fast, sensitive and label-free fluorescence sensing platform for evaluating the acetylcholinesterase (AChE) activity was developed based on the inner filter effect (IFE) between black phosphorus quantum dots (BP QDs) and 2-nitro-5-thiobenzoate anion (TNB). BP QDs were successfully synthesized from bulk BP through a facile top-down route by sonication-assisted solvothermal method. The as-prepared QDs exhibit green fluorescence with a relative high quantum yield, good photostability and relative pH resistance. 5,5′-Dithiobis-(2-nitrobenzoic acid) (DTNB), as a well-known Ellman’s reagent, can react with thiol groups to form TNB. Based on the IFE between BP QDs and TNB, a sensitive and label-free sensing platform was developed for fluorescent measurement of thiols. As a proof-of-concept application, the platform was further applied in the evaluation of AChE activity by using acetylthiocholine as the substrate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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