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The effects of cyclohexanhexol-derived synthetic ice blockers (SIBs) including 1,3-cyclohexanediol (1,3-CHD) and 1,4-cyclohexanediol (1,4-CHD) on chilled or frozen Yunnan semi-fine wool ram spermatozoa were systematically assessed and compared in this study. The collected semen was pooled and equally divided into two parts. In the first part, in order to evaluate the effect of SIBs on chilled spermatozoa before freezing, the semen was divided into nine groups according to the concentrations of SIBs (0, 25 mM, 50 mM, 100 mM, or 200 mM). In the second part, the treatment of semen was similar to the first part. However, except for the cooling and equilibrating procedures, spermatozoa were preserved in liquid nitrogen for one month. Motility, moving velocity, acrosome status, membrane integrity, and mitochondrial membrane potential (MMP) of frozen/thawed spermatozoa were determined using a computer-assisted spermatozoa analysis system (CASAS), fluorescence staining, and flow cytometry. The data indicated that when the concentrations of SIBs were less than or equal to 100 mM, there is no significant difference among the treating groups as concern spermatozoa motility and moving velocity following equilibration at 5 °C. However, when the concentrations of SIBs were increased to 200 mM, the motility and moving velocity of chilled ram spermatozoa were significantly decreased compared to the control spermatozoa ( P P P |
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