Effects of Storing Pig Ovaries at 4 or 20°C for Different Periods of Time on the Morphology and Viability of Pre-Antral Follicles

Autor:	Christiani Andrade Amorim, J.R. Dobrinsky, Carolina Madeira Lucci, Sônia Nair Báo, L L Schreier, G. M. Machado
Rok vydání:	2006
Předmět:	medicine.medical_specialty Morphology (linguistics) medicine.medical_treatment Ovarian tissue Ovary Biology Antral follicle Endocrinology medicine.anatomical_structure Internal medicine medicine Animal Science and Zoology Ovarian follicle Saline Biotechnology
Zdroj:	Reproduction in Domestic Animals. 42:76-82
ISSN:	0936-6768
Popis:	The purpose of this study was to evaluate the effect of cooling ovarian tissue on pig pre-antral follicles. Ovaries were maintained in saline solution (0.9%) at 4 or 20 degrees C for 6, 12 or 18 h. After storage, pre-antral follicles were morphologically evaluated. While primordial follicles were not affected by the storage, the percentage of morphologically normal growing follicles was significantly reduced in ovarian tissue stored at 20 degrees C for 12 or 18 h. To test the viability of stored follicles, growing follicles isolated from ovaries stored at 4 degrees C for 18 h and at 20 degrees C for 6 h were cultured for 3 days. Follicles stored in either condition presented the same growth pattern in vitro as fresh follicles. We conclude that storage of pig ovaries at 4 degrees C for up to 18 h or at 20 degrees C for up to 6 h does not affect the morphology of growing follicles or their ability to grow in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c953258b84cc4a596d55462d73fe4fbf https://doi.org/10.1111/j.1439-0531.2006.00734.x Zobrazit plný text záznamu Plný text