Incidence of enterotoxigenic Staphylococcus aureus in milk and Egyptian artisanal dairy products
| Autor: | Nagah Mohammed Saad Maharik, Antonio Valero, Ahmed Abdel-Hameid Ahmed, Sahar Mahmoud Kamal |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Meticillin
business.industry medicine.drug_class Incidence (epidemiology) 010401 analytical chemistry Antibiotics food and beverages 04 agricultural and veterinary sciences Drug resistance Enterotoxin Biology Food safety medicine.disease_cause 040401 food science 01 natural sciences 0104 chemical sciences 0404 agricultural biotechnology Antibiotic resistance Staphylococcus aureus medicine Food science business Food Science Biotechnology medicine.drug |
| Zdroj: | Food Control. 104:20-27 |
| ISSN: | 0956-7135 |
| DOI: | 10.1016/j.foodcont.2019.04.017 |
| Popis: | Staphylococcus aureus (S. aureus) is one of the most concerning foodborne pathogens due to its ability to produce wide varieties of heat-stable enterotoxins. Hence, this study was designed to investigate the prevalence of the enterotoxigenic S. aureus strains and their enterotoxins in milk and in some representative artisanal Egyptian dairy products. Additionally, the prevalence of Methicillin-Resistant S. aureus (MRSA; antibiotic resistant strain) was considered throughout the study. Three-hundred and fifty samples (n = 350) were randomly collected from Assuit city in upper Egypt, including market cow's milk, yoghurt, Domiati cheese, Kareish cheese, Ras cheese, cooking butter and small-scale ice cream. The identification of the S. aureus strains was done using conventional biochemical method (catalase test, egg yolk reaction test, anaerobic utilization of mannitol and coagulase test) and PCR. Furthermore, Staphylococcal enterotoxins genes (SEA and SED) were detected by PCR while the classical enterotoxins were examined in 30 samples of Ras cheese and small-scale ice cream using ELISA technique. S. aureus was isolated from 142 out of 350 (41%) [95% CI: 35.4–45.7%] examined samples at levels of up to 5.79 log10 cfu/ml or g. The prevalence of S. aureus was significantly (P |
| Databáze: | OpenAIRE |
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