Radical-scavenging activity of penicillin G, ampicillin, oxacillin, and dicloxacillin
| Autor: | Hassan Y. Aboul-Enein, Irena Kruk, Paweł Berczyński, Aleksandra Kładna |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species Antioxidant DPPH medicine.medical_treatment Radical 010401 analytical chemistry Biophysics 01 natural sciences Dicloxacillin 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Chemistry (miscellaneous) medicine Organic chemistry Light emission Hydroxyl radical Trolox medicine.drug Nuclear chemistry |
| Zdroj: | Luminescence. 32:434-442 |
| ISSN: | 1522-7235 |
| DOI: | 10.1002/bio.3199 |
| Popis: | The aim of this study was to characterize the antioxidant activity of penicillin G (PG), ampicillin (AMP), oxacillin (OX) and dicloxacillin (DOX) through their reactivity towards reactive oxygen species (superoxide anion radical, O2•; hydroxyl radical, HO• ; peroxyl radical, ROO• ; hydrogen peroxide, H2 O2 ; DPPH• ) using various in vitro antioxidant assays with chemiluminescence (CL) and spectrophotometry as measurement techniques. In hydroxyl radical assays , PG, OX and AMP were found to inhibit the CL signal arising from the Fenton-like reaction in a dose-dependent manner with IC50 = 0.480 ± 0.020 mM, IC50 = 0.569 ± 0.021 mM, and IC50 = 0.630 ± 0.019 mM, respectively. The highest reactivity of PG among the tested penicillins towards the HO radical was confirmed in the deoxyribose degradation assay. In the ABAP-derived ROO radical assay, the radical-scavenging ability of the test penicillins was in the following order: AMP > PG > DOX > OX. The number of reduced DPPH radicals by the drugs tested was |
| Databáze: | OpenAIRE |
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