| Autor: |
Anupama Rao, Baken Lyu, Ishrat Jahan, Anna Lubertozzi, Gao Zhou, Frank Tedeschi, Eckhard Jankowsky, Junsu Kang, Bryan Carstens, Ken Poss, Kedryn Baskin, Joseph Aaron Goldman |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.08.15.502524 |
| Popis: |
The eIF4E family of translation initiation factors bind 5’ methylated caps and act as the limiting-step for mRNA translation. The canonical eIF4E1A is required for cell viability, yet other related eIF4E families exist and are utilized in specific contexts or tissues. Here, we describe a family called Eif4e1c for which we find roles during heart development and regeneration in zebrafish. The Eif4e1c family is present in all aquatic vertebrates but is lost in all terrestrial species. A core group of amino acids shared over 500 million years of evolution forms an interface along the protein surface, suggesting Eif4e1c functions in a novel pathway. Deletion ofeif4e1cin zebrafish caused growth deficits and impaired survival in juveniles. Mutants surviving to adulthood had fewer cardiomyocytes and reduced proliferative responses to cardiac injury. Ribosome profiling of mutant hearts demonstrated changes in translation efficiency of mRNA for genes known to regulate cardiomyocyte proliferation. Althougheif4e1cis broadly expressed, its disruption had most notable impact on the heart and at juvenile stages. Our findings reveal context-dependent requirements for translation initiation regulators during heart regeneration. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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