Collagen-specific T cells are not downregulated following treatment with 17β-estradiol in CIA (129.39)
| Autor: | Kary Latham, Karen Whittington, Edward Rosloniec |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 178:S225-S225 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.178.supp.129.39 |
| Popis: | Increases in estrogen levels in rheumatoid arthritis (RA) patients result in amelioration of RA symptoms. Similarly, administration of estrogen (17β-estradiol, or E2) decreases incidence of collagen-induced arthritis (CIA), a well-established murine model for RA. Our laboratory established that this decrease was associated with a change in the isotype of antibodies produced to type II collagen (CII) following immunization with CII from predominantly IgG2a to IgG1. In order to determine if E2-driven changes in the T cell response regulate this isotype switch, we focused on E2’s effect on the function of CD4+ CII-specific T cells. After immunization with CII, analysis of CII-specific T cells using MHC class II tetramers showed that their numbers increased slightly in E2-treated mice at 10 days post-immunization compared to controls. There were no significant differences in expression of activation markers (CD69, CD44, or CD62L) by CII-specific T cells between groups. Surprisingly, analysis of cytokine expression by qPCR following cell sorting of the tetramer+ cells indicated that the CII-T cells from E2-treated mice expressed higher levels of IL-17 mRNA than did CII-T cells from controls. Levels of other proinflammatory cytokines such as IL-2, IFN-γ, and IL-6 were similar to those found in tetramer+ cells from controls. These results suggest that CII-T cells do not directly mediate E2’s protective effect in CIA. |
| Databáze: | OpenAIRE |
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