Metformin Inhibits Propofol-Induced Apoptosis of Mouse Hippocampal Neurons HT-22 Through Downregulating Cav-1
| Autor: | Yi Zhang, Jiafeng Sun, Gu Tianyu, Jianyun Ge, Yulin Huang, Lei Yao, Xu Liu, Mengmeng Cai, Lin Liu, Jie Song |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pharmacology endocrine system diseases medicine.diagnostic_test Chemistry nutritional and metabolic diseases Pharmaceutical Science Stimulation Hippocampal formation Neuroprotection Metformin 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Downregulation and upregulation Western blot Apoptosis 030220 oncology & carcinogenesis Drug Discovery medicine Propofol medicine.drug |
| Zdroj: | Drug Design, Development and Therapy. 14:1561-1569 |
| ISSN: | 1177-8881 |
| DOI: | 10.2147/dddt.s229520 |
| Popis: | Objective To elucidate the neuroprotective function of metformin in suppressing propofol-induced apoptosis of HT-22 cells. Methods HT-22 cells were treated with 0, 10 or 100 μmol/L propofol, followed by determination of their proliferative ability. Subsequently, changes in proliferation and apoptosis of propofol-treated HT-22 cells induced with metformin were assessed. Apoptosis-associated genes in HT-22 cells were detected by Western blot. At last, regulatory effects of Cav-1 on propofol and metformin-treated HT-22 cells were examined. Results Propofol treatment dose-dependently decreased proliferative ability and increased apoptosis ability in HT-22 cells, which were partially blocked by metformin administration. Upregulated Bcl-2 and downregulated Bax were observed in propofol-treated HT-22 cells following metformin administration. In addition, Cav-1 level in HT-22 cells was regulated by metformin treatment. Notably, metformin reversed propofol-induced apoptosis stimulation and proliferation decline in HT-22 cells via downregulating Cav-1. Conclusion In our study, we found that propofol could induce apoptosis of HT-22 cells and metformin could rescue the apoptosis effect regulated by propofol. Then, we found that metformin protects propofol-induced neuronal apoptosis via downregulating Cav-1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|