A comprehensive study of biocompatibility of detonation nanodiamonds

Autor: Sergei V. Ageev, Vladimir V. Sharoyko, Robert Ishmukhametov, Demid A. Kirilenko, Anatolii A. Meshcheriakov, Andrey V. Petrov, Mikhail A. Galkin, Konstantin N. Semenov, Gregory M. Berdichevskiy, Alexei V. Nashchekin, Sofia D. Martynova, Lubov V. Vasina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Molecular Liquids. 332:115763
ISSN: 0167-7322
DOI: 10.1016/j.molliq.2021.115763
Popis: The article describes a complex study of detonation nanodiamonds (DND) aqueous dispersions. In this research, DND sample was characterised by means of IR, NMR spectroscopy, TEM, thermogravimetric analysis, size distribution, and ζ-potentials. It was shown that DND sample includes several surface groups, mainly hydroxylic, carboxylic, and carbonyl ones. Dynamic light scattering results revealed that in the concentration range C = 0.002–0.3 wt%, DND nanoparticles size is equal to 55 ± 5 nm. It was demonstrated that DND possessed weak antiradical activity, had an inhibitory effect on F1F0-ATPase activity, almost did not affect platelet aggregation, formed a stronger complex with human serum albumin (HSA) in subdomain IB (digitoxin, Kb = 20.0 ± 2.4 l·g−1) and a less strong complex in subdomain IIA (warfarin, Kb = 3.7 ± 0.1 l·g−1), inhibited the esterase activity of HSA, DND dispersions (C = 0.0012–0.15 wt%) revealed genotoxic effect towards PBMCs, did not affect cellular proliferation in the experiment with HEK293 cell line, did not reveal cytotoxic effect up to 0.01 wt%. Using DFT and MD approaches allowed us to perform a simulation of interaction between DND nanoparticle and water molecules.
Databáze: OpenAIRE
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