Reducing the background to silver-stained polyacrylamide gels after two-dimensional electrophoresis

Autor:	S. Peter Spragg, Robert Amess
Rok vydání:	1986
Předmět:	Silver stain Electrophoresis chemistry.chemical_compound Chromatography Two dimensional electrophoresis Chemistry Clinical Biochemistry Polyacrylamide Biochemistry Analytical Chemistry
Zdroj:	Electrophoresis. 7:444-446
ISSN:	1522-2683 0173-0835
DOI:	10.1002/elps.1150071003
Popis:	A high background found after silver staining of polyacrylamide gels following two-dimensional electrophoresis and generated from the cathode in the second-dimensional separation has been investigated. Material is formed which is electrophoretically mobile and apparently derived from the buffer components. It behaves like a peptide as far as silver staining. The intensity of this background was reduced by treatment of the second-dimensional gel with a photographic fixing agent prior to silver staining. Moreover, spots which previously appeared negatively stained were changed by the treatment and the result was a general improvement in the contrast.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c8eb219fcd5a9bcbce84e06af87b6397 https://doi.org/10.1002/elps.1150071003 Zobrazit plný text záznamu Plný text