Immobilization of Tyrosinase on Ion Exchange Resins and Application of Removal of a Phenol Compound
| Autor: | Erika Satoh, Kenjiroh Nishi, Jun-ichi Kawagoe, Yohko Ichimura, Ayumi Tamura, Kazunori Yamada |
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| Rok vydání: | 2008 |
| Předmět: |
Materials science
Polymers and Plastics Materials Science (miscellaneous) Tyrosinase Phenol Compound technology industry and agriculture Chitosan chemistry.chemical_compound chemistry Covalent bond parasitic diseases Polymer chemistry Chemical Engineering (miscellaneous) Molecule Epichlorohydrin Ion-exchange resin General Environmental Science Nuclear chemistry Carbodiimide |
| Zdroj: | KOBUNSHI RONBUNSHU. 65:104-107 |
| ISSN: | 1881-5685 0386-2186 |
| DOI: | 10.1295/koron.65.104 |
| Popis: | We investigated the covalent immobilization of mushroom tyrosinase on an anion-exchange resin with a water-soluble carbodiimide and removal of a phenol compound, p-cresol, by using two columns packed with immobilized tyrosinase and chitosan beads. An increase in the immobilization time led to an increase in the amount of immobilized tyrosinase. Tyrosinase molecules were covalently immobilized on chitosan beads through the covalent multipoint attachment. Tyrosinase immobilized on anion-exchange resins for 72 h had an almost constant activity for 5-time repetitive p-cresol removal experiments. p-Cresol was also effectively removed by use of chitosan beads prepared with epichlorohydrin in a shortened treatment time. These results made clear that the stability and reusability of tyrosinase was improved by covalent immobilization on the resins and that p-cresol was effectively removed by using two columns packed with tyrosinase-immobilized resins and chitosan beads. |
| Databáze: | OpenAIRE |
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