The cloning of hrnt-1 using a combination of cdna library screening with biotin-labeled probe and rapid amplification of cDNA ends

Autor:	Kai-tai Zhang, De-chang Wu, Ying-chun Hu, Gang Li
Rok vydání:	2001
Předmět:	Cloning Genetics clone (Java method) Cancer Research Expressed sequence tag Oncology Rapid amplification of cDNA ends Accession number (library science) cDNA library GenBank Complementary DNA Biology Molecular biology
Zdroj:	Chinese Journal of Cancer Research. 13:166-170
ISSN:	1000-9604
Popis:	Objective: To clone the human counterpart of rat ZA73, EST cloned from rat tracheal epithelial (RTE) neoplastic transformed cell model induced by (a-particles radiation by using mRNA differential display. Methods: According to the sequence of rat ZA73, a probe was biotin-labeled to screen human cDNA library, and then the gene sequence was extended by RACE (rapid amplification of cDNA ends). Result: Human gene HRNT-1 (GenBank Accession Number: AF223393) is 4.256 kb in length, with an ORF located in the region between 254 and 3013 bp. 5′ UTS (untranslated sequences) is 253 bp, 3′ UTS is 1243 bp. Conclusion: The combination of cDNA library screening with biotin-labeled probes and RACE is an effective method to clone full-length cDNA, especially for sequences longer than 2 kb.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c8da691ddbc87cef0f567a109ab2f3f3 https://doi.org/10.1007/bf02983877 Zobrazit plný text záznamu Full text from SpringerLink