Fluorescent probe for Fe(III) with high selectivity and its application in living cells
| Autor: | Si-Jie Zhang, Kun-Peng Wang, Yu-Bing Zhang, Zhi-Qiang Hu, Qi Zhang, Yang Lei, Wen-Jun Zheng, Shaojin Chen, Ju-Peng Chen |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Aqueous solution 010405 organic chemistry Metals and Alloys Analytical chemistry 010402 general chemistry Condensed Matter Physics Alkali metal Hydrazide 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Rhodamine chemistry.chemical_compound chemistry Stability constants of complexes Acetyl chloride Materials Chemistry Electrical and Electronic Engineering Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 252:1140-1145 |
| ISSN: | 0925-4005 |
| Popis: | An easily available novel fluorescent probe has been synthesized by reacting rhodamine hydrazide and 2-(thiophen-2-yl)acetyl chloride. Upon mixed with Fe 3+ in aqueous solution, the dramatic enhancement of fluorescence intensity as well as the color change of the solution were observed. Significantly, the probe displayed highly selective and sensitive recognition ability toward Fe 3+ with a limit of detection (LOD) down to 10 −7 M. The Job’s plot indicated the formation of 1:1 complex between the probe and Fe 3+ , and the complexation stability constant can be calculated as 2.95 × 10 4 M −1 . The presence of common coexisting alkali, alkaline earth, and transition metal ions showed small or no interference to the detection of Fe 3+ . All these unique features make it particularly favorable for cellular Fe 3+ imaging applications. The confocal fluorescent images demonstrated that the probe was cell-permeable and could be used for highly sensitive monitoring of Fe 3+ levels in living cells with satisfying resolution. |
| Databáze: | OpenAIRE |
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