Confocal imaging of exine as a tool for grass pollen analysis
| Autor: | Guy Cox, Allan S. Jones, Anya Salih, Diana Bass |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Poa pratensis
Setaria biology Optical sectioning Confocal food and beverages Plant Science biology.organism_classification medicine.disease_cause Phalaris aquatica law.invention Confocal microscopy law Pollen Botany otorhinolaryngologic diseases medicine Paspalum dilatatum Ecology Evolution Behavior and Systematics |
| Zdroj: | Grana. 36:215-224 |
| ISSN: | 1651-2049 0017-3134 |
| Popis: | A new method which utilises confocal optical imaging has been developed which can be expected to improve grass pollen analysis. Confocal microscopy, in reflection mode, was used to examine the exine morphology of unacetolysed pollen grains from the following species of common wild grasses: Paspalum dilatatum, Setaria gracilis, Bromus catharticus, Daclylis glomerata, Lolium perenne, Poa pratensis, and Phalaris aquatica. Variations in the surface texture patterns, similar to those hitherto only seen by scanning electron microscopy, were visualised. In contrast to the latter method, specimen preparation for this confocal microscopy based technique was characterised by its simplicity, permitting the use of fresh and chemically untreated pollen grains. This confocal imaging technique, with its capacity for optical sectioning of specimens, offered the additional advantage of allowing the examination of the sub‐surface exine layers as well as the surface morphology of the pollen grains. Furthermore, three‐dimens... |
| Databáze: | OpenAIRE |
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