Interaction of Surfactants and Polymer-Grafted Lipids with a Plant Lipid Transfer Protein, LTP1
| Autor: | Denise Sy, Jean-Paul Douliez, Françoise Vovelle, Didier Marion |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Telechelic polymer Ligand Cationic polymerization Surfaces and Interfaces Polymer Condensed Matter Physics chemistry.chemical_compound Monomer chemistry Bromide Critical micelle concentration Polymer chemistry Electrochemistry Organic chemistry General Materials Science Ethylene glycol Spectroscopy |
| Zdroj: | Langmuir. 18:7309-7312 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la020163w |
| Popis: | To probe the effect of charge and ligand polar head, we report on the interaction between a plant lipid transfer protein (LTP1) and cationic surfactants, anionic and nonionic polymer grafted lipids, cetyl trimethylammonium bromide (CTAB), dihexadecyl dimethylammonium bromide (DHDAB), dimyristoyl phosphatidylethanolamine poly(ethylene glycol) (DMPE−PEG2000), and a telechelic polymer (PEG-dioleate), respectively. As revealed by intrinsic protein fluorescence, these ligands were loaded in the protein hydrophobic cavity with an affinity of about 2 μM except for the telechelic polymer for which the affinity was 10 times lower. These results suggest that the protein can bind ligands whatever the nature, size, and charge of their polar head. Moreover, the protein is shown to bind monomers in solution in the case of CTAB since the titration experiment was performed at a concentration lower than its critical micelle concentration (cmc). Altogether, this reveals that the protein does not require any interface to wr... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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